DNA Sequencing Facilities
Center for Mammalian Genetics
University of Florida
Important Notice: The Center for Epigenetics DNA Sequencing Core Facility has
ceased opertions effective August 15, 2011. We recommend that DNA sequencing samples
be sent to the UFL Interdisciplinary Center for Biochnology Research (ICBR)
DNA sequencing service is available to all
research groups within the Center for Mammaliam Genetics (CMG). The
sequencing facilities comprises of ABI Prism 377XL and
3130XL Automated DNA Sequencers.
CMG DNA sequencing facilities provide the following services
- The sale of ABI Prism BigDye
terminator cycle sequencing ready reaction kit with AmpliTaq DNA
polymerase, FS. Kits are for sale as a 10, 25, 50 or 100 reaction and
one reaction cost $6.75. Please contact Akilah McDaniel
or call 352-392-3054 for purchansing information.
- Loading of DNA sequencing reaction on ABI DNA sequencer,
generation of DNA sequencing data file, and troubleshot of DNA
sequencing. Average lenght of DNA sequencing is 700 nucleotides. Average
turnaround time is 3 working days. The cost for running DNA sequencing
reaction is $2.50(For CMG members) or $3.00(For Non-CMG member).
- Analysis of DNA sequencing files using Factura Feature
Identification Software. Factura identifies specified vector and
ambiguity ranges, rectriction sites, and a specified confidence range.
It also identifies multiples base positions with codes described by the
International Union of Biochemists (IUB codes) based on a user-defined
threshold. No charge for this service.
- GeneScan run. GeneScan allow to size and quantify DNA fragments.
This service cost $100 per gel and up to 34 samples or $3.00 for per
sample.(can be run in one gel. You can label different DNA fragments
with up to three different color flourescent dyes, you can use a fourth
dye color to label an internal DNA standard. Please inquire about this
- Plese summit your samples using our
- Bring samples to sixth floor of the Dental Tower Building to room
number D6-19(Address: CMG DNA Sequencing Core Facility, 1600 SW Archer
Rd, PO Box 100275).
- Data files can be download using CMG FTP server
from our serve (using WS_FTP, your usename and password)
ftp://dnasequencecore.cmg.health.ufl.edu or bring your floppy or Zip
Use the following protocols for DNA sequencing:
The ABI PRISM 377XL
DNA sequencer will automatically analyse DNA molecules labelled with
any of four different fluorescent dyes. After samples are loaded onto
the system's vertical gel system, they undergo electrophoresis, laser
detection, and computer analysis. Electrophoresis separation can be
viewed on-screen in real-time, and final data can be printed in a
variety of formats. This equipment provides a rapid and inexpensive
method for deriving DNA sequence from both cloned DNA inserts and PCR
377XL sequencing chemistries:
- Four types of sequencing chemistry are now available for the ABI
377XL equipment. Taq polymerase Dye Primer chemistry, Dye Terminator
chemistry, dRhodamine Terminator chemistry and the latest Big Dye
Terminator chemistry. Selection of the correct chemistry for a given
sequencing strategy can greatly enhance your results.
3130XL sequencing chemistries:
- Two types of sequencing chemistry are now available for the ABI
3130XL equipment. dRhodamine Terminator
Program to view and edit ABI DNA sequencing files
- Applications for viewing ABI files created by 377 or 3130.
Versions for both Mac and PC systems are available.
For further information contact the DNA Sequencing Assistant
Scientist at 352-392-7835, Jing-Da
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